What is the required incubation temperature and time for the Total Coliform Membrane Filtration (MF) technique using m-Endo medium?

• 35 ± 0.5 °C for 22 to 24 hours
• 44.5 ± 0.2 °C for 24 hours
• 20 °C for 5 days
• 121 °C for 15 minutes